Seting off the molecular techniques for the genetics caracterization of Apis mellifera in Cuba
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Abstract
Due to its importance in food production, the honey bee (Apis mellifera) is subjected to breeding programs and management. To this must be taken into account their genetic potential; related to its ability to grow, develop, reproduce and disease resistance. This is determined by genetic, specific to each individual and their parents inherited factors that are not manageable and are expressed depending on environmental conditions. The scenario becomes more complex when the progeny are so great. Then, it is necessary a genetic management for better production performance of these stocks. In Cuba, this species is handled in a mass selection program of progenitors. This program is guided for the genetic characterization using molecular markers. Given this, the present work aims the implementation of molecular techniques for the genetic characterization of A. mellifera in Cuba. The detection of microsatellite loci was efficiently achieved using DNA extracted by Chelex´s method using the thorax muscle of the bee, subsequent sequence amplification by PCR with Ready to Go Taq commercial mixture and finally, conventional polyacrylamide gel electrophoresis without major modifications.
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