Application of a methodology for determinate the floral origin of pollen collected by Apis mellífera l., Cuba
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Abstract
Fresh pollen is a raw food, rich in nutrients and easily assimilated. The palynological test methods for pollen differentiation and classification are laborious and expensive. Considering this, we evaluate a simple methodology, without the use of chemical treatments that would allow the differentiation of pollen grains, determine the representativeness of each floral species and typify pollen in monofloral, polifloral or bifloral. Six samples of fresh pollen from the Artemisa and Mayabeque provinces during the years 2018 and 2019 were analyzed. Corbicular pollen balls were separated from each sample into groups defined by color. The microscopic analysis of the pollen grains allowed differentiating 12 pollen types, of which the real palm pollen grain was present in the six samples. Pollen grains belonging to the Arecaceae, Asteraceae, Mimosaceae and Fabaceae families were observed in at least three of the samples. From the weight percentage of each color group, three samples were classified in bifloral and three in monofloral or real palm. The families identified are listed in Cuba as important polyniferous sources for bees
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References
Alaux, C., F. Ducloz, D. Crauser and Y. Le Conte (2010). "Diet effects on honeybee immunocompetence." Biology letters 6: 562-565.
Barreto, A. (1990). "Botánica de las leguminosas." Instituto de Ecología y Sistemática. La Habana, Cuba.
Basualdo, M., S. Barragán and K. Antúnez (2014). "Bee bread increases honeybee haemolymph protein and promote better survival despite of causing higher N osema ceranae abundance in honeybees." Environmental microbiology reports 6: 396-400.
Brodschneider, R. and K. Crailsheim (2010). "Nutrition and health in honey bees." Apidologie 41: 278-294.
Erdtman, G. (1960). "The acetolysis method-a revised description." Sven Bot Tidskr 54: 516564.
Estévez, Y., G. R. Castro, T. M. Peña and T. Y. Díaz (2011). "Implementación del ensayo: análisis polínico para productos apícolas."
Fonnegra, R. (1989). "Introducción a la palinología." Medellín, Colombia: Universidad de Antioquia.
García, D. L. Q., M. d. l. L. A. Sánchez and S. A. Vizarro (2002). "Morfología de los granos de polen de la familia Polemoniaceae del estado de Querétaro, México." Polibotánica: 57-66.
Louveaux, J., A. Maurizio and G. Vorwohl (1978). "Methods of melissopalynology." Bee world 59: 139-157.
Machado, S. and L. Sotolongo (2000). Polen de las principales plantas melíferas de Cuba, Apiciencia.
Marchini, L. C., V. D. A. dos Reis and A. C. d. C. C. Moreti (2006). "Composição físico-química de amostras de pólen coletado por abelhas Africanizadas Apis mellifera (Hymenoptera: Apidae) em Piracicaba, Estado de São Paulo." Ciência Rural 36: 949-953.
Maurizio, A. (1975). "Microscopy of honey." Honey: A Comprehensive Survey. E. Crane, ed.
Montenegro, G., M. Gomez and G. Avila (1992). "Importancia relativa de especies cuyo polen es utilizado por Apis mellifera en el área de la Reserva Nacional Los Ruiles, VII región de Chile." Acta botánica malacitana 17: 167-174.
Montenegro, G., M. Gomez, P. Estay and X. Ortega (2011). "Polen apícola–Calidad de la colmena para polinización y diferenciación del polen según origen botánico mediante ensayo palinológico." Norma Chilena NCh3255. c2011. Instituto Nacional de Normalización, Santiago, Chile. Versión Final Comité–Septiembre.
Montoya-Pfeiffer, P. M., D. León-Bonilla and G. Nates-Parra (2014). "Pollen catalog for Apis mellifera honey from coffee regions in the Sierra Nevada de Santa Marta, Magdalena, Colombia." Revista de la Academia Colombiana de Ciencias Exactas, Físicas y Naturales 38:
-384.
Odoux, J.-F., D. Feuillet, P. Aupinel, Y. Loublier, J.-N. Tasei and C. Mateescu (2012). "Territorial biodiversity and consequences on physico-chemical characteristics of pollen collected by honey bee colonies." Apidologie 43: 561-575.
Pérez, A. (2007). "Manual de apicultura." La Habana, Cuba: Agrinfor.
Rinderer, T. E. and K. Dell Elliott (1977). "Worker honey bee response to infection with Nosema apis: influence of diet." Journal of Economic Entomology 70: 431-433.
Rinderer, T. E., W. C. Rothenbuhler and T. A. Gochnauer (1974). "The influence of pollen on the susceptibility of honey-bee larvae to Bacillus larvae." Journal of invertebrate pathology 23: 347-350.
Roulston, T. a. H., J. H. Cane and S. L. Buchmann (2000). "What governs protein content of pollen: pollinator preferences, pollen–pistil interactions, or phylogeny?" Ecological monographs 70: 617-643.
Sauri Duch, E. (1998). "Plantas melíferas más importantes de la península de Yucatan (México) y principales características de su polen. II. Catálogo de las características de las plantas y de sus granos de polen." Apiacta 33: 56-64.
Sayas Rivera, R. and L. Huamán Mesía (2009). "Determinación de la flora polinífera del valle de Oxapampa (Pasco-Perú) en base a estudios palinológicos." Ecología aplicada 8: 53-59.
Soldevilla, C. G., P. C. González, P. A. Teno and E. D. Vilches (2007). Manual de calidad y gestión de la Red Española de Aerobiología.
Telleria, I. and M. Sarasola (2002). "Análisis de polen corbicular recolectado durante los años 2002 y 2003 en los colmenares de estudio eco-etológico de Oñati y Goizueta. http://www.euskadi.eus/contenidos/informe_estudio/eco_etológico_abejas/es_doc/adjuntos/analisis_polínico. pdf
Thibault, M. (2016). Le pollen apicole: ses propriétés et ses utilisations thérapeutiques, Université de Lorraine.